Conference Day One
Thursday, November 7, 2024
7:45 am Check In & Morning Coffee
8:45 am Chair’s Opening Remarks
Focusing the Complexities of Lipid Nanoparticles to Comprehensively Characterize Structural Properties
9:00 am KEYNOTE ADDRESS: Examining the Quantification Metrics Available To Characterize Size, PDI & Zeta Potential of Lipid Nanoparticles
Synopsis
- Outlining the technology available to quantify the size and zeta potential of the lipid nanoparticle within a sample
- Analyzing size dispersity with the target site central to discussion to ensure maximal uptake through the pores
- Creating novel quantitative biophysical characterization techniques, magnifying size and structure, as well as dynamic properties
9:30 am Debunking the Assumptions of Protein Corona Formation Around the Lipid Nanoparticle To Understand the Components Responsible for the Association
Synopsis
- Characterizing the protein corona to understand the composition of protein and how this relates to LNP outcome
- Researching the lipid components which correlate with specific protein corona formations
- Magnifying oxidation of lipids as a potential cause of an advanced protein corona cascade
10:00 am Session Reserved for BA Sciences
Synopsis
10:30 am Morning Refreshments & Speed Networking
Collating In-Depth Pre-Clinical Characterization Data Packages to Inform LNP Formulation
11:30 am Developing a Thorough Process To Analyze & Screen the Lipid Components of the Lipid Nanoparticle To Ensure Stable Formulations
Synopsis
- Exploring the essential factors in the lipid screening process to enhance characterization
- Addressing the current obstacles in the lipid screening and discovery field to support lipid nanoparticle formulation
- Utilizing advanced automated and high-throughput microfluidic technologies for efficient lipid screening
12:00 pm Screening the Profile of Surface Functionalized Lipid Nanoparticles To Understand the Impact on Delivery
Synopsis
- Implementing a thorough method to characterize the variety of targeting moieties available to lipid nanoparticles
- Adequately characterizing the chemical structures and density of the surface markers
- Relaying the characterization data to grasp the impact the surface structure has on delivery outcomes
12:30 pm Mapping the Relationships Between Critical Quality Attributes To Define the Data Points of True Importance
Synopsis
- Outlining the current definition of ‘critical quality attributes’ to prioritize characterization efforts
- Digging deeper to uncover the ‘why’ behind certain readouts and what this means to the functionality of the lipid nanoparticle
- Interconnecting critical quality attributes to comprehend the dynamic relationship between such measurements
1:00 pm Session Reserved for Partner Presentation: Anton Paar
Synopsis
1:15 pm Lunch & Networking
Maximizing Control Through Standardized Sample Preparation & Aligning Process Development Requirements
2:15 pm Reconsidering the Structural & Dynamic Measurements Responsible To Extensively Quantify Stability
Synopsis
- Brainstorming methods to quantify the stability profile of the lipid nanoparticles with varied formulation and manufacturing processes
- Pinpointing the key morphological properties of lipid nanoparticles which play a role in the stability outcome of the nanoparticle
- Considering the dynamic properties which can trigger instability and installing methods to measure these interactions over a prolonged period
2:45 pm Spotlighting the Structural & Dynamic Properties of the Lipid Nanoparticle which are Predictive of Storage & Temperature Requirements
Synopsis
- Leveraging the early analytics required to indicate the manufacturing steps in later development, including temperature and storage controls
- Characterizing the factors which contribute to temperature instability within lipid nanoparticles
- Working collaboratively with process development teams to allow for analytics to indicate the process
3:15 pm Panel Discussion: Benchmarking Gold Standard Analytical Sample Preparation Standards To Mitigate Variability
Synopsis
- Preparing a lipid nanoparticle sample which is representative of the true sample to avoid discrepancies
- Establishing a process to ensure the entirety of the sample is being quantified to ensure maximal control
- Standardizing sample preparation, material handling and method robustness to increase the reliability of analytical practices
4:00 pm Afternoon Refreshments & Networking
Managing the Diverse Interactions with LNP Components & the Payload, Indicating the Endosomal Release Outcome
4:30 pm Characterizing the Lipid to Payload Interaction within the Nanoparticle, Providing Clarity in the Encapsulation Dynamics
Synopsis
- Focusing the dynamics behind payload to lipid association to understand the degree of interaction within the lipid nanoparticle
- Quantifying the dispersity of payload across a lipid nanoparticle to understand the location of the drug substance
- Reviewing the differences in characterization across varying payloads, from RNA to CRISPR to cell therapies
5:00 pm Overcoming the Bottlenecks Associated with Co-Formulated Lipid Nanoparticles to Successfully Differentiate the Encapsulated Material
Synopsis
- Introducing the formulative approach to develop a co-formulated lipid nanoparticle
- Showcasing the challenges faced when characterizing the internal dynamics of 2 payloads of varying size and compositions
- Generating metrics to accurately record the ratios of payloads within a lipid nanoparticle