Advanced Surface & Tissue Analytics for Triphosphate Stabilized PEGFree LNPs
- Demonstrate that removing PEG-lipid from a four-component LNP system does not compromise colloidal stability, RNA integrity over nine months, or in vivo hepatic potency, provided surface charge is controlled through polyanion binding
- Microscale thermophoresis (MST) was originally developed for fluorescence-labelled protein binding and here adapted to directly quantify small-molecule ion binding to LNP surfaces, establishing anion–lipid affinity as a measurable parameter
- Deploy MALDI-MS imaging to spatially resolve ionizable lipid distribution across hepatic microanatomy at 20 μm resolution, linking formulation to intrahepatic biodistribution patterns